What is Nitric Oxide?

Nitric oxide (N:O) is known as the anti-aging molecule and is one of the most important molecules naturally produced in humans. This simple molecule regulates cellular communication by controlling oxygen and nutrient delivery to every cell in the body. When you don’t produce enough Nitric Oxide, your cells can’t maintain optimal health and start to malfunction.

How Loss of Nitric Oxide Affects Your Skin

Cells malfunction (age) when they do not get enough oxygen and nutrients. Skin cells are no different. Healthy cells have sufficient collagen and are hydrated. Unhealthy cells lose collagen, lose hydration and fine lines and wrinkles appear.

Nitric Oxide helps the body provide nutrients and oxygen so the skin cells can restore their function. This includes collagen deposition, improved hydration, the loss of fine lines and wrinkles, and improvement to the tone, quality, and texture of our skin.

Healthy skin cells are like little buckets filled with nutrients, oxygen and water.  As you age, your body loses its ability to produce enough Nitric Oxide. Your little buckets spring holes allowing the nutrients and moisture to leak from the cells. Unhealthy skin cells require constant replenishment of nutrients and hydration just to maintain. The older you get, the less Nitric Oxide your body makes, the less nitric oxide your body makes the more your skin cells malfunction (age), the more skin cells malfunction the more your skin ages and wrinkles as you get older. Loss of nitric oxide production is part of the normal aging process.

Nitric Oxide Discoveries Win Nobel Prize

Nitric Oxide is so important, it was named “Molecule of the Year” by Science Magazine in 1992 and in 1998, a Nobel Prize in Physiology or Medicine was awarded to the three US scientists responsible for its discovery.

The Nobel Prize in Physiology & Medicine 1998
“For… discoveries concerning nitric oxide as a signaling
molecule in the cardiovascular system”

Groundbreaking Skincare Backed by 20 Years of Scientific Research and
Peer-Reviewed Published Clinical Studies.

Annals of Plastic and Reconstructive surgery

Read the Clinical Aging Skin Study
Published in the Annals of Plastic and Reconstructive Surgery

“The Utilization of a Topical Nitric Oxide Generating Serum in the Aging Skin Population”

Study in International Journal of Pharmaceutica Analytica Acta

Read the Clinical Active Acne Study
Published in the International Journal of Pharmaceutica Analytica Acta

“The Utilization of a Nitric Oxide Generating Serum in the Treatment of Active Acne Scarred Patients”

‘The discovery of nitric oxide and its function is one of the most important in the history of cardiovascular medicine.

Valentine Fuster, M.D.
1998 President of the American Heart Association
Head of Cardiology at Mount Sinai Hospital in Manhattan

There are two ways the body normally makes NO. One is from the amino acid L-arginine. The enzyme that converts L-arginine into nitric oxide becomes dysfunctional. In fact, we lose about 10-12% of nitric oxide production per decade so by the time we reach the age of 40, we have only about half the nitric oxide production we had when we were in our late teens and early twenties. This is due to many factors including aging, oxidative stress, poor diet, lack of physical exercise, smoking, diabetes, high sugar diet, certain drug therapy, etc.

The other pathway to make nitric oxide is from nitrate and nitrite found naturally in some foods.  However, this is dependent upon eating enough green leafy vegetables that contain sufficient nitrate.  It is estimated that a typical Western diet only contains about 150mg of nitrate. We need about 300-400 mg per day to make sufficient nitric oxide.  Therefore, most people are not getting sufficient nitric oxide from their diet. Furthermore, the use of antiseptic mouthwash, antibiotics and antacids completely shuts down nitric oxide production from this pathway.  There are over 200,000,000 Americans that use antiseptic mouthwash every day and hundreds of millions more that take antibiotics and antacids. This puts most Americans deficient in this critical molecule.

Ten Facts about Nitric Oxide (NO)

  1. Nitric oxide is one of the most important molecules produced in the human body.
  2. NO is a potent vasodilator that keeps your arteries open and blood flowing to every cell in your body.
  3. Nitric oxide is made by every cell in your body; however, production declines by 10-12% per decade starting in the early 20s.  By age 40-50, we make only 50%.
  4. Loss of NO production is associated with the normal aging process.
  5. Prescription drugs, such as proton pump inhibitors, NSAIDS, antibiotics, antiseptic mouthwash and cholesterol-lowering statin drugs block the production of NO.
  6. Most people do not know what nitric oxide is or why it is critical that they employ strategies to restore nitric oxide.
  7. NO has been shown to dilate blood vessels and recruit new blood vessels, improving circulation to regions as it is produced or supplied.
  8. NO has potent anti-inflammatory properties that help combat free radicals that damage cells and cause premature aging.
  9. NO is responsible for cellular energy production and cell metabolism that keeps cells healthy and functional.
  10. Due to diet, lifestyle and many drug therapies, most Americans are deficient in nitric oxide.

Creating Nitric Oxide
on the Surface of the Skin

Lab testing confirms incredible results.

Infrared thermography can be used to detect changes in radiation. The amount of radiation emitted by an object increases with temperature. Therefore, thermography allows one to see variations in temperature. The image above shows baseline temperature prior to application of Pneuma Nitric Oxide Skin Serum.

The image above is the thermogram of same person 4 minutes after application of Pneuma Nitric Oxide Skin Serum to face, next and chest. The increase in nitric oxide production is apparent.

4-Minute Progression After Application

Nitric Oxide (NO) is considered one of the most important molecules naturally produced in humans. This simple molecule controls oxygen and nutrient delivery to every cell in the body, it regulated cellular communication. All of which affects the signs of aging. In fact, this molecule is so important it was named “Molecule of the Year” by
Science Magazine in 1992 and in 1998 a Nobel Prize in Physiology or Medicine was awarded to the three US scientists responsible for its discovery. Loss of nitric oxide production is part of the normal aging process.


Berkowitz, D.E., et al., Arginase reciprocally regulates nitric oxide synthase activity and contributes to endothelial dysfunction in aging blood vessels. Circulation, 2003. 108(16): p. 2000-6.

Taddei, S., et al., Age-related reduction of NO availability and oxidative stress in humans. Hypertension, 2001. 38(2): p. 274-9.

Egashira, K., et al., Effects of age on endothelium-dependent vasodilation of resistance coronary artery by acetylcholine in humans. Circulation, 1993. 88(1): p. 77-81.

Gerhard, M., et al., Aging progressively impairs endothelium-dependent vasodilation in forearm resistance vessels of humans. Hypertension, 1996. 27(4): p. 849-53.

Lundberg, J.O., et al., Nitrate, bacteria and human health. Nat Rev Microbiol, 2004. 2(7): p. 593-602.

Hord, N.G., Y. Tang, and N.S. Bryan, Food sources of nitrates and nitrites: the physiologic context for potential health benefits. Am J Clin Nutr, 2009. 90(1): p. 1-10.

Kapil, V., et al., Inorganic nitrate supplementation lowers blood pressure in humans: role for nitrite-derived NO. Hypertension, 2010. 56(2): p. 274-81.

Kapil, V., et al., Physiological role for nitrate-reducing oral bacteria in blood pressure control. Free Radic Biol Med, 2013. 55: p. 93-100.

Bryan, N.S., et al., Nitrite is a signaling molecule and regulator of gene expression in mammalian tissues. Nat Chem Biol, 2005. 1(5): p. 290-7.

Bryan, N.S. and J.L. Ivy, Inorganic nitrite and nitrate: evidence to support consideration as dietary nutrients. Nutr Res, 2015. 35(8): p. 643-54.

Bryan, N.S., K. Bian, and F. Murad, Discovery of the nitric oxide signaling pathway and targets for drug development. Front Biosci, 2009. 14: p. 1-18.

Nitric oxide production in the skin is a normal process that declines with age 1,2.

  1. Nitric Oxide. 2004 Jun;10(4):179-93.
  2. Hypertension, 2001. 38(2): p. 274-9

Indeed, loss of production of nitric oxide leads to advanced aging and the appearance of fine lines and wrinkles along with poor complexion and tone of the skin.